Total rna extraction from tissues for microrna and target. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation. Homogenates were subjected to a single freezethaw cycle to aid cell lysis and then cleared by centrifugation at 10,000 x g for 5 min at 4 c. For highthroughput disruption of biological samples pdf 89kb. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation in cell culture. Tissuelyser adapter set 2 x 96 from qiagen selectscience. Tissuelyser ii tissuelyser ii delivers medium to highthroughput disruption of up to 2 x 96 samples of tissue, plant material, yeast or bacteria in less than 5 minutes. The tissuelyser ii is an integral part of qiagens complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. Laboratory equipment qiagen qiacube connect safety instructions and quick start manual 56 pages for use with qbase laboratory equipment qiagen tissuelyser ii user manual 48 pages. Qiagen s position as the market leader for sample and assay technologies is based on more than 25 years of experience, strong relationships with customers, o. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. Do not continue to use the tissuelyser ii or tissuelyser accessory if it is damaged. Use of the tissuelyser ii with nonqiagen adapters has been tested by other.
Tissueruptor tissuelyser lt tissuelyser ii throughput samplesrun 1 up to 12 up to 48 or 192 disruption technology rotorstator bead mill bead mill disruption time 30 seconds 40 seconds to 5 minutes 20 seconds to 5 minutes table 1. Safety information 6 tissuelyser ii user manual 042010 with accessories approved by qiagen see section 3. The following guidelines can be used for disruption and homogenization of. Cuttingedge solutions for your life science, applied testing. Guidelines for disruption and homogenization of tissues using the tissuelyser ii 37. Centrifuge the plate at room temperature for 6 min at 4500 x g. Under these conditions, qiaquick spin kits can be stored for up to 12 months without showing any reduction in performance and quality. The tissuelyser ii simultaneously disrupts multiple biological samples through high speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads.
Low cost and nontoxic genomic dna extraction for use in. The tissuelyser ii and tissuelyser adapter set 2 x 24 allow highthroughput, rapid, and effective disruption of 48 biological samples in 24 minutes. Lowcost dna extraction for use in tilling and ecotilling assays. Cignal lenti reporter handbook sabiosciences, a qiagen. Objective to develop and adapt a method for low cost and nontoxic genomic dna extraction for use in molecular marker studies. Does anyone use the tissuelyser ii qiagen equipment to disrupt. Tissuelyser handbook university of texas at arlington. For efficient disruption of up to 48 or 192 samples in parallel on the tissuelyser ii.
Sample tubes rb 2 ml x 2 ml sample tubes, for tissuelyser lt qiagen 990381 reagent dx qiagen 19088 allprep kit mini qiagen 80204 allprep kit micro qiagen 80284 fisherbrand textured nitrile gloves large fisher 27005853 ice bucket green fisher 1167636 wet ice in house na na nuclease free 2. Storage the tissuelyser should be stored upright in a dry environment at room temperature 1525c. Simultaneous purification of genomic dna and total rna, including small rnas from ffpe cores, using the allprep dnarna ffpe kit. Homogenization and disruption with the tissuelyser ii gives results comparable to using rotorstator homogenization. Page 5 l low cost dna extraction for use in tilling and ecotilling assays, version 1. Tissuelyser ii user manual university of texas at arlington. There are minor cosmetic scratches on the top plate, but nothing serious. Print bookmark share more for disruption of up to 12 biological samples. Tissuelyser ii medium to highthroughput disruption. Prepared by bernhard hofinger, owen huynh and brad till. Does anyone use the tissuelyser ii qiagen equipment to.
Place a stainless steel bead 5 mm diameter in the microcentrifuge tube containing the sample. See the tissuelyser lt handbook or the tissuelyser handbook. Complete disruption and homogenization may not always be possible. It has been stored under unfavorable conditions for a prolonged period.
It has been subjected to severe transport stresses. Qiagen qiagen purification kits compatible with qiagen disruption systems. Qiagen qiacube dnarna purification sample preparation bostonind 09 duration. Welcome to qiagen digital insights labcorp uses qci and hgmd to improve identification and interpretation of genetic variants within inhereited diseases. Convenient and secure disruption process adapter sets optimized for highthroughput disruption wide range of accessories available reproducible results with difficulttolyse tissues qiagen tissuelyser ii. Qiaquick spin kits should be stored dry at room temperature 1525c. This easytouse instrument automates purification of dna, rna, and protein from 196 samples. Qiagen disruption systems for a range of throughputs automated solutions from sample to result. Reorient plates so that the side that was closest to the machine body is now farthest from it and shake again at speed 20 hz for 10 min. Can the tissuelyser by qiagen be used to lyse cell culture rather than using trizol and the like. Equipnet is constantly receiving used qiagen equipment through our exclusive contracts with our clients. Qiagen offers advanced and innovative solutions for all of your assay and sample equipment needs.
Tissuelyser lt handbook for disruption of up to 12 biological samples. Do not continue to use the tissuelyser ii or tissuelyser. Tissuelyser ii, supplied by qiagen, used in various techniques. Disruption and homogenization using the tissuelyser lt or tissuelyser ii. Dna adsorbs to the dneasy membrane in the presence of high concentrations. Can the tissuelyser by qiagen be used to lyse cell culture. The tissuelyser lt is fully compatible with qiagen manual sample preparation kits. Qiagen tissuelyser ii sample disruption 11843 youtube. Tissuelyser ii qiagen bioz ratings for lifescience research. In the european union, in accordance with the specific weee recycling requirements and where a replacement product is being supplied by qiagen, free.
Tissuelyser adapter set, 2 x 24 or tissuelyser adapter set 2 x 96 available separately 85300 tissuelyser adapter set 2 x 24. Alternatively, a grinding jar set can be used to process large samples. Apr 28, 2017 tissuelyser lt tissuelyser lt is a small bead mill that enables low to mediumthroughput sample disruption, of up to 12 samples at the same time. Before using the tissuelyser ii, it is essential you read this user manual carefully.
Servicing of the tissuelyser ii should only be performed by qiagen or an. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus. Laboratory equipment qiagen tissuelyser ii user manual 48 pages. We stiil use pestle and mortar which is very timeconsuming and we would like to speed. Automated nucleic acid purification on the qiacube purification of dna using the dneasy powersoil pro kit can be automated on the qiacube. For disruption of up to 48 or 192 biological samples. Tissuelyser ii user manual 052016 7 it or the line power cord appears to be damaged.
Dna isolation with qiagen genomictips this protocol applies to. The qiagen tissuelyser lt sample disruptor provides quick and efficient disruption in up to 12 samples. Disrupt and homogenize the ffpe core using the tissuelyser ii. The tissuelyser ii is an integral part of qiagen s complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. If the tissuelyser ii is not in use, switch it off at the power switch. Forceps are to be sterilized in fine science tools heat block at. For longer storage, qiaquick spin kits can also be stored at 28c, but in this case the buffers should be redissolved before use. Qiagen, provides free recycling of its weeemarked electronic equipment in. Advanced settings appendix f advanced settings advanced run settings are available to adjust specific functions on the qiacube ht. All instructions and protocols in this handbook apply to both the tissuelyser ii.
The tissuelyser ii is also compatible with qiagen manual sample. May 2016 tissuelyser ii user manual san francisco state. What is the alternative for tissuelyser for rna extraction. This small bead mill has high throughput with highspeed shaking for the disruption or homogenization of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. Place the tube containing the homogenate on the benchtop at room temperature 1525c for 5 min. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation, such as the qiasymphony sp. The innovative qiacube uses advanced technology to process qiagen spin columns. What is the alternative for tissuelyser for rna extraction from muscle samples. Place the tubes in the tissuelyser adapter set 2 x 24. Place tubes, or collection tube rack, in the respective tissuelyser adapter and operate the instrument according to instructions in the handbook. Qiagen focuses on breaching great success and development in their company to better serve their consumers.
Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. All instructions and protocols in this handbook apply to both the tissuelyser ii and the tissuelyser. Low cost and nontoxic genomic dna extraction for use in molecular marker studies. Adapter sets for flexible sample throughput 2 x 24, 2 x 96. Quality of barley genomic dna extractions using silica powder and different combinations of selfmade. Tissuelyser lt handbook qiagen gel electrophoresis. Jun 02, 2014 add the appropriate volume of buffer rlt see table 8, and homogenize by passing the lysate at least 5 times through a blunt 20gauge needle fitted to an rnase free syringe.
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